Strickler-Berg calculator

Calculate:

NA

Extinction Coefficient

Quantum Yield

Fluorescence Lifetime

Input parameters and spectral data:

Extinction Coefficient (in M-1 cm-1):

Quantum Yield (0-1):

Fluorescence Lifetime (in ns):

Refractive index (n):

1.330 (H2O)

1.365 (in cells)

Other

NA

Absorbance&Emission spectrum:

Fluorescein Abs/Em data

mTurquoise2 Abs/Em data

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Wavelength (in nm), Absorbance, Emission

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About

About the Strickler-Berg calculator

This app uses spectra and spectroscopic parameters as input to calculate either the EC, QY or tau by using the Stricler-Berg equation (Strickler and Berg, 1962). Two out of the three (EC, QY, tau) parameters are required as input. The spectral data must be provided as three columns that respectively contain 1:Wavelength (in nm), 2:Absorbance, 3:Emission. An example file in csv format is available here

Credits

The script is based on a Matlab macro generated by Marten Postma

This app is created and maintained by Joachim Goedhart (@joachimgoedhart) and Marten Postma